Diclazuril Testing in Feed and Food

Diclazuril is a synthetic coccidiostat used primarily in poultry to control infections caused by Eimeria species. It belongs to the triazine class of compounds and differs structurally and mechanistically from ionophores.
It is typically incorporated into feed at low inclusion levels and may be used as part of rotation or shuttle programmes to maintain effective coccidiosis control.

Diclazuril is commonly used in broiler production systems where precise dosing is critical due to its low inclusion rate. Its activity at low concentrations makes accurate premix formulation and homogeneous mixing essential.
Because it may be used in combination with or following other coccidiostats, production sequencing and contamination control remain important considerations.

Although generally well tolerated at authorised levels, deviations in inclusion rate or unintended exposure of non-target feed may result in regulatory or production issues.
Potential concerns include:
‣ Incorrect formulation
‣ Cross-contamination during manufacture
‣ Carry-over into withdrawal feed
‣ Residue compliance requirements
Regulatory frameworks define authorised inclusion levels and establish compliance expectations to protect animal performance and consumer safety.

Given its low dosing levels, analytical confirmation is essential to verify correct inclusion and to monitor carry-over between batches.
LC-MS/MS methods enable reliable quantification of diclazuril in feed matrices, supporting quality control and regulatory compliance programmes.